NbActiv4 (For Primary Neuron Cultures)

$264.95
SKU: NB4
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NbActiv4® neuron culture medium is an improved version of Neurobasaltm / B27tm.  NbActiv4 grows neurons with twice as many synapses and up to 8x higher spike rates than Neurobasal/B27.

NbActiv4 was optimized for maximum spike rates by addition of 3 new components to Neurobasal/B27: creatine, estrogen and cholesterol.  Measures of respiration indicated that neurons in NbActiv4 were under less metabolic stress and operated more efficiently under demand loading from glutamate. NbActiv4 is a convenient and useful serum-free neuron culture medium for electrophysiology, developmental synaptic plasticity, neuronal network analyses, pharmacology, toxicology and drug discovery. Synapses and spiking activity develop earlier, starting at 1 week in NbActiv4 compared to Neurobasal/B27 (Brewer et al. 2008. Neuron network activity scales exponentially with synapse density.  J. Neural Eng., in press) Brewer,GJ., Boehler, MD; Jones, TT, Wheeler, BC. 2008. NbActiv4 medium for neurons increases synaptic densities and network spike rates on multielectrode arrays compared to Neurobasal/B27.

J. Neurosci. meth. 170:181-187 (PM 18308400)

 Strategies for switching to NbActiv4® from Neurobasal/B27

NbActiv4 contains all the ingredients in Neurobasal + B27 +Glutamax (all Invitrogen).  It can be stored at 4 °C for up to 6 months if unopened.  It should not be frozen.  Both media (Neurobasal/B27 and NbActiv4) are designed for use at 37 °C in a 5% CO2 incubator.  Neurons are subject to less oxidative stress if the oxygen is also regulated at 9%.  To evaluate NbActiv4 in the context of current experiments, we suggest a side-by-side comparison.  For new lines of study, experiments can begin directly with NbActiv4.  Beginning cultures in Neurobasal/B27 followed by a switch to NbActiv4 shows no indication of being better than use of NbActiv4 from the start. One-Half medium changes with incubator temperature equilibrated medium should be performed at least once a week, with more frequent changes at cell densities above 300 cells/mm2.

Click here for our Primary Neuronal Culturing Protocol.

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