Now, BrainBitsÂ® is a distributor of Lipofectamineâ„¢ 2000 Transfection Reagent.Â Â When combined with our specially formulated BrainBitsÂ® Transfection Media, Lipofectamineâ„¢ 2000 is ideal for hard to transfect primary neurons. We compared three popular transfection reagents and Lipofectamineâ„¢ 2000 outperformed the competition.Â Â
â€¢Â Â Â Â Â Â Â Â Â Â Â Â Optimized for use with BrainBitsÂ® tissue, dissociated, and adherent neurons.
â€¢Â Â Â Â Â Â Â Â Â Â Â Â Our transfection protocol prevents toxicity.
â€¢Â Â Â Â Â Â Â Â Â Â Â Â A wide range of available volumes to meet transfection requirements while preventing reagent waste.
When combined with the BrainBits Dissociated Cell Culturing Kit, you have everything you need to start a culture from freshly dissociated embryonic tissue for your transfection experiments.
Dissociated cells in 2 mL of HibernateÂ® EB Complete.
12 mL of NbActiv1â„¢ media for initiating a culture of primary rat or mouse neurons.
A 9â€ silanized, fire polished pipette and bulb.
A pair of 50 Âµg/mL poly-D-lysine coated 15 mm glass coverslips.
1 Vial of Lipofectamine 2000 Transfection Reagent
1 Vial of BrainBits Transfection Media
A dissociated cell and transfection protocol.
Click here to view FAQ
Dissociated Cell Protocol
Please note: All fresh tissue/kits MUST be shipped via Priority Overnight. All orders must be received the day before the specified ship date. Click here for our printable Rat Tissue Order Formor Mouse Tissue Order Form. Please contact us with any questions