BrainBits® Dissociated Cell Culturing Kit The BrainBits Dissociated Cell Culturing Kit contains everything you need to start a culture from single cell suspensions mechanically dissociated from freshly dissected E18 rat tissue. Dissociated cells are stored in 2 mL of Hibernate® EB Complete, which helps to maintain cell viability for up 14 days when stored at 4ºC.
One order of Neuroprogenitors is from one brain which will provide over 1 million neurospheres. Neurospheres are grown in progenitor medium (12 ml provided) on uncoated low adhesion plastic (corning pdf). Differentiation requires tissue culture plastic or glass coated with poly-d-lysine. Microsurgically dissected regions from E18 rat is provided along with media to initiate your culture.