Microsurgically dissected hippocampus tissue from E18 mouse are provided in order to initiate a culture of primary mouse neurons All orders must be placed the day before the specified dissection dated media are provided to initiate a culture of primary rat astroglia.
One order is from one brain which will provide over 1 million neurospheres. Neurospheres are grown in progenitor medium (12 ml provided) on uncoated low adhesion plastic (corning pdf). Differentiation requires tissue culture plastic or glass coated with poly-d-lysine. Microsurgically dissected regions from E18 mouse is provided along with media to initiate your culture.