One order is from one brain which will provide over 1 million neurospheres. Neurospheres are grown in progenitor medium (12 ml provided) on uncoated low adhesion plastic (corning pdf). Differentiation requires tissue culture plastic or glass coated with poly-d-lysine. Microsurgically dissected regions from E18 mouse is provided along with media to initiate your culture.

Hibernate® is a nutrient medium for the maintenance of neural tissue or cells in ambient carbon dioxide levels (US Patent 6,180,404). BrainBits® is the sole supplier of custom Hibernate Medium.

Hibernate® is a nutrient medium for the maintenance of neural tissue or cells in ambient carbon dioxide levels (US Patent 6,180,404). BrainBits® is the sole supplier of custom Hibernate Medium.

Adult Rat Septum Tissue Fresh adult Septum tissue and media are provided to initiate a culture.

Fixed CNS Slide One unstained slide - E18 Sprague Dawley rat hippocampal or cortical primary neurons after 21 days in culture. This item takes 3-4 weeks to receive. Neurons are grown on a glass coverslip, fixed, and mounted on a glass microscope slide.

Dulbecco's Phosphate Buffered Saline (DPBS) is a buffer solution containing sodium chloride, sodium phosphate, potassium chloride and potassium phosphate and is used to maintain cell culture media in the physiological pH range of 7.0 to 7.6. DPBS can serve as an irrigating, transporting or diluting fluid while maintaining cell tonicity and viability for a limited period of time. Formulations with calcium and magnesium are generally used as transport media or for reagent preparation.

MAP2 / CAMKII Neuronal Coverslips Immunostained E18 Sprague Dawley rat hippocampal or cortical neurons after 14 days in culture stained for MAP2 (microtubule associated protein 2) and CaMKII (calcium / calmodulin-dependent protein kinases II). Stained red (568 nm excitation for CAMKII, green (488 nm excitation) for MAP2. Neurons are grown on a glass coverslip, fixed, and mounted on a glass microscope slide.

Adult Mouse Combined Cortex, Hippocampus, and Ventricular Zone Primary Neuron Tissue Fresh adult Combined Cortex, Hippocampus, and Ventricular Zone brain tissue and media are provided to initiate a culture of primary mouse neurons.

Introducing our new cardiomyocyte transport medium. This medium has been developed along side our Cardiomyocyte cell line.

Pretreated Polystyrene Culture Plates These polystyrene plates are tissue culture-treated for optimum cell attachment and growth and pretreated with 50 μg/mL PDL for 24 hours. Optically clear plates are compatible with most automatic diluters, readers, and pipettors. Raised well rims, lid rings, and recessed areas prevent cross-contamination and reduce evaporation. Uniform wall thickness ensures distortion-free well bottoms. Well positions are labeled with alphanumeric markings. Gamma radiation sterilized. Certified nonpyrogenic.

E18 Thalamus Fresh E18 tissue from Sprague Dawley rat and media are provided to initiate a culture of Thalamus.

Whole Heart Dissociated Cells: Cell Culturing Kit Introducing our new cardiomyocyte dissociated culturing kit. Each contains the components that you will need to start a successful culture of cardiomyocytes. Each Kit contains the following: •One vial of dissociated heart from a Sprague Dawley rat or C57 mouse, in 2 ml of NbCardio™ -Transport Medium. •12ml of NbCardio™ Growth and NbCardio™ Maintenance •One fire polished pipette with latex bulb. •One pair of gelatin coated coverslips. •Protocol. Click here for our Dissociated Cardiomyocyte Cell Culture Kit Protocol